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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using FEM1C Recombinant antibody (83536-1-RR)

    WB analysis of HepG2 using 83536-1-RR (same clone as 83536-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83536-1-RR (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83536-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using FEM1C Recombinant antibody (83536-1-RR)

    FC experiment of U2OS using 83536-1-RR (same clone as 83536-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Fem1c Recombinant Antibody (83536-1-RR, Clone:240126G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83536-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83536-1-RR

    Affinity and Kinetic Characterization of 83536-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83536-1-RR against Human FEM1C were performed. The affinity constant is 0.896 nM.

FEM1C Recombinant monoclonal antibody, PBS Only

FEM1C Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83536-1-PBS
Clone No.240126G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83536-1-PBS targets FEM1C in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5099

Product name: Recombinant human FEM1C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 315-617 aa of BC028369

Sequence: PDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFKRCINLWKYALDMQQSNLDPLSPMTASSLLSFAELFSFMLQDRAKGLLGTTVTFDDLMGILCKSVLEIERAIKQTQCPADPLQLNKALSIILHLICLLEKVPCTLEQDHFKKQTIYRFLKLHPRGKNNFSPLHLAVDKNTTCVGRYPVCKFPSLQVTAILIECGADVNVRDSDDNSPLHIAALNNHPDIMNLLIKSGAHFDATNLHKQTASDLLDEKEIAKNLIQPINHTTLQCLAARVIVNHRIYYKGHIPEKLETFVSLHR

相同性解析による交差性が予測される生物種
Full Name fem-1 homolog c (C. elegans)
Calculated molecular weight 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC028369
Gene Symbol FEM1C
Gene ID (NCBI) 56929
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ96JP0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.

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