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Species Reactivity

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  • Featured Product
  • KD/KO Validated

FEM1B Polyclonal antibody

FEM1B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19544-1-AP

Synonyms

F1A alpha, F1AA, fem 1 homolog b (C. elegans), FEM1 beta, FEM1B, FIAA, KIAA0396, Protein fem 1 homolog B

Validation Data Gallery

Applications:
  • WB analysis of PC-3 using 19544-1-AP

    WB analysis of PC-3 using 19544-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse pancreas using 19544-1-AP

    WB analysis of mouse pancreas using 19544-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 19544-1-AP

    WB analysis of mouse testis using 19544-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW264.7 using 19544-1-AP

    WB analysis of RAW264.7 using 19544-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 19544-1-AP

    IHC staining of human pancreas cancer using 19544-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 19544-1-AP

    IHC staining of human pancreas cancer using 19544-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 19544-1-AP

    IHC staining of mouse pancreas using 19544-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 19544-1-AP

    IHC staining of mouse pancreas using 19544-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 19544-1-AP

    IHC staining of human pancreas cancer using 19544-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 19544-1-AP

    IF Staining of PC-3 using 19544-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 19544-1-AP (FEM1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, mouse pancreas tissue, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19544-1-AP targets FEM1B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name fem-1 homolog b (C. elegans)
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberNM_015322
Gene symbol FEM1B
Gene ID (NCBI) 10116
RRIDAB_10644294
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FEM1B, also named F1AA and KIAA0396, belongs to the fem-1 family. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. It is involved in glucose homeostasis in pancreatic islets.

Protocols

Product Specific Protocols
WB protocol for FEM1B antibody 19544-1-APDownload protocol
IHC protocol for FEM1B antibody 19544-1-APDownload protocol
IF protocol for FEM1B antibody 19544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

A Cellular Mechanism to Detect and Alleviate Reductive Stress.

Authors - Andrew G Manford
  • KD Validated
View Article
humanWB

J Am Chem Soc

Discovery of a Covalent FEM1B Recruiter for Targeted Protein Degradation Applications

Authors - Nathaniel J. Henning
View Article