Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of PC-3 using 19544-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 19544-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 19544-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 19544-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 19544-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 19544-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 19544-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 19544-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 19544-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-3 using 19544-1-AP
Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (19544-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

Featured Product
KD/KO Validated

FEM1B Polyclonal antibody

FEM1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19544-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FEM1-beta, FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	PC-3 cells, mouse pancreas tissue, mouse testis tissue
Positive IHC detected in	human pancreas cancer tissue, mouse pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 1 publications below

Product Information

19544-1-AP targets FEM1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	fem-1 homolog b (C. elegans)
Calculated molecular weight	70 kDa
Observed molecular weight	70 kDa
GenBank accession number	NM_015322
Gene Symbol	FEM1B
Gene ID (NCBI)	10116
RRID	AB_10644294
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UK73
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FEM1B, also named F1AA and KIAA0396, belongs to the fem-1 family. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. It is involved in glucose homeostasis in pancreatic islets.

Protocols

Product Specific Protocols
IF protocol for FEM1B antibody 19544-1-AP	Download protocol
IHC protocol for FEM1B antibody 19544-1-AP	Download protocol
WB protocol for FEM1B antibody 19544-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell A Cellular Mechanism to Detect and Alleviate Reductive Stress. Authors - Andrew G Manford KD Validated View Article
human	WB	J Am Chem Soc Discovery of a Covalent FEM1B Recruiter for Targeted Protein Degradation Applications Authors - Nathaniel J. Henning View Article
	WB	Cell Structural basis and regulation of the reductive stress response Authors - Andrew G Manford View Article
human	WB,IF	Mol Cell Reactive oxygen species control protein degradation at the mitochondrial import gate Authors - Rachael McMinimy View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of PC-3 using 19544-1-AP

WB analysis of mouse pancreas using 19544-1-AP

WB analysis of RAW264.7 using 19544-1-AP

WB analysis of mouse testis using 19544-1-AP

IHC staining of human pancreas cancer using 19544-1-AP

IHC staining of human pancreas cancer using 19544-1-AP

IHC staining of mouse pancreas using 19544-1-AP

IHC staining of mouse pancreas using 19544-1-AP

IHC staining of human pancreas cancer using 19544-1-AP

IF Staining of PC-3 using 19544-1-AP

FEM1B Polyclonal antibody

FEM1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A Cellular Mechanism to Detect and Alleviate Reductive Stress.

Discovery of a Covalent FEM1B Recruiter for Targeted Protein Degradation Applications

Structural basis and regulation of the reductive stress response

Reactive oxygen species control protein degradation at the mitochondrial import gate