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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FDX1 Recombinant antibody (82957-2-RR)

    WB analysis using 82957-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82957-2-RR (FDX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FDX1 Recombinant antibody (82957-2-RR)

    WB analysis of HEK-293 using 82957-2-RR

    WB result of FDX1 antibody (82957-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using FDX1 Recombinant antibody (82957-2-RR)

    WB analysis using 82957-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82957-2-RR (FDX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FDX1 Recombinant antibody (82957-2-RR)

    IHC staining of human stomach cancer using 82957-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82957-2-RR (FDX1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using FDX1 Recombinant antibody (82957-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82957-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82957-2-RR (FDX1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FDX1 Recombinant antibody (82957-2-RR)

    IF Staining of HepG2 using 82957-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (82957-2-RR, Clone: 230196E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using FDX1 Recombinant antibody (82957-2-RR)

    FC experiment of A431 using 82957-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.4 ug FDX1 Recombinant antibody (82957-2-RR, Clone:230196E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HepG2 cells using FDX1 Recombinant antibody (82957-2-RR)

    FC experiment of HepG2 using 82957-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FDX1 Recombinant antibody (82957-2-RR, Clone:230196E9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), or 0.25 ug FDX1 Recombinant antibody (82957-2-RR, Clone: 230196E9) (green). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82957-2-RR

    Affinity and Kinetic Characterization of 82957-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82957-2-RR against Human FDX1 were performed. The affinity constant is 17.3 nM.

  • Featured Product
  • KD/KO Validated

FDX1 Recombinant monoclonal antibody

FDX1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82957-2-RR
Clone No.230196E9
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Ferredoxin-1, FDX, ADX, 230196E9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, PC-3 cells, U-87 MG cells, U-251 cells, HK-2 cells
Positive IHC detected inhuman stomach cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

82957-2-RR targets FDX1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3301

Product name: Recombinant human FDX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-184 aa of BC017063

Sequence: MAAAGGARLLRAASAVLGGPAGRWLHHAGSRAGSSGLLRNRGPGGSAEASRSLSVSARARSSSEDKITVHFINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGCQICLTKSMDNMTVRVPETVADARQSIDVGKTS

相同性解析による交差性が予測される生物種
Full Name ferredoxin 1
Calculated molecular weight 184 aa, 19 kDa
Observed molecular weight14 kDa
GenBank accession numberBC017063
Gene Symbol FDX1
Gene ID (NCBI) 2230
RRIDAB_3670701
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10109
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

Protocols

Product Specific Protocols
WB protocol for FDX1 antibody 82957-2-RRDownload protocol
IHC protocol for FDX1 antibody 82957-2-RRDownload protocol
IF protocol for FDX1 antibody 82957-2-RRDownload protocol
FC protocol for FDX1 antibody 82957-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Metab Brain Dis

Edaravone dextranol alleviates ferroptosis, Cuproptosis, and blood-brain barrier damage after acute cerebral infarction

Authors - Guimin Jin
View Article