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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FDX1 Recombinant antibody

FDX1 Recombinant Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230196B12

Cat no : 82957-1-RR

Synonyms

Adrenal ferredoxin, Adrenodoxin, mitochondrial, ADX, FDX, FDX1, ferredoxin 1, Hepatoredoxin, LOH11CR1D

Validation Data Gallery

Applications:
  • WB analysis using 82957-1-RR

    WB analysis using 82957-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82957-1-RR (FDX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82957-1-RR

    IF Staining of HepG2 using 82957-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (82957-1-RR, Clone: 230196B12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 82957-1-RR

    FC experiment of HepG2 using 82957-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human FDX1 (82957-1-RR, Clone:230196B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of A431 using 82957-1-RR

    FC experiment of A431 using 82957-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human FDX1 (82957-1-RR, Clone:230196B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HEK-293 cells, PC-3 cells
Positive IF detected inHepG2 cells
Positive FC detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

82957-1-RR targets FDX1 in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FDX1 fusion protein Ag3301 相同性解析による交差性が予測される生物種
Full Name ferredoxin 1
Calculated molecular weight 184 aa, 19 kDa
Observed molecular weight14 kDa
GenBank accession numberBC017063
Gene symbol FDX1
Gene ID (NCBI) 2230
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

Protocols

Product Specific Protocols
WB protocol for FDX1 antibody 82957-1-RRDownload protocol
IF protocol for FDX1 antibody 82957-1-RRDownload protocol
FC protocol for FDX1 antibody 82957-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols