Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, rat testis tissue, mouse testis tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34182-1-AP targets FDX1 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag41687 Product name: Recombinant mouse FDX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 64-184 aa of BC017063 Sequence: DKITVHFINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGCQICLTKSMDNMTVRVPETVADARQSIDVGKTS 相同性解析による交差性が予測される生物種 |
| Full Name | ferredoxin 1 |
| Calculated molecular weight | 184 aa, 19 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC017063 |
| Gene Symbol | FDX1 |
| Gene ID (NCBI) | 14148 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P46656 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ferredoxin 1 (FDX1) is a small mitochondrial protein and recent studies have shown that FDX1 plays an important role in tumor cuproptosis. FDX1 expression is significantly downregulated in HCC tissues. FDX1 downregulation promotes HCC cell proliferation, invasion in vitro and growth, metastasis in vivo. In addition, FDX1 affects metabolism of HCC cells and is associated with autophagy (PMID: 39128228). FDX1 is localized to the mitochondria and contains a 64-amino-acid mitochondrial transit peptide that is cleaved during post-translational processing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FDX1 antibody 34182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |