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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FDPS Polyclonal antibody (16129-1-AP)

    WB analysis using 16129-1-AP (same clone as 16129-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16129-1-AP (FDPS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using FDPS Polyclonal antibody (16129-1-AP)

    WB analysis of A431 using 16129-1-AP (same clone as 16129-1-PBS)

    WB result of FDPS antibody (16129-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDPS transfected A431 cells. This data was developed using the same antibody clone with 16129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using FDPS Polyclonal antibody (16129-1-AP)

    WB analysis of rat liver using 16129-1-AP (same clone as 16129-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 16129-1-AP (FDPS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human cerebellum tissue using FDPS Polyclonal antibody (16129-1-AP)

    WB analysis of human cerebellum using 16129-1-AP (same clone as 16129-1-PBS)

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16129-1-AP (FDPS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16129-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using FDPS Polyclonal antibody (16129-1-AP)

    IP experiment of mouse liver using 16129-1-AP (same clone as 16129-1-PBS)

    IP result of anti-FDPS (IP:16129-1-AP, 4ug; Detection:16129-1-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16129-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FDPS Polyclonal antibody, PBS Only

FDPS Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 16129-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16129-1-PBS targets FDPS in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9115

Product name: Recombinant human FDPS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 68-419 aa of BC010004

Sequence: NGDQNSDVYAQEKQDFVQHFSQIVRVLTEDEMGHPEIGDAIARLKEVLEYNAIGGKYNRGLTVVVAFRELVEPRKQDADSLQRAWTVGWCVELLQAFFLVADDIMDSSLTRRGQICWYQKPGVGLDAINDANLLEACIYRLLKLYCREQPYYLNLIELFLQSSYQTEIGQTLDLLTAPQGNVDLVRFTEKRYKSIVKYKTAFYSFYLPIAAAMYMAGIDGEKEHANAKKILLEMGEFFQIQDDYLDLFGDPSVTGKIGTDIQDNKCSWLVVQCLQRATPEQYQILKENYGQKEAEKVARVKALYEELDLPAVFLQYEEDSYSHIMALIEQYAAPLPPAVFLGLARKIYKRRK

相同性解析による交差性が予測される生物種
Full Name farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase)
Calculated molecular weight 419 aa, 48 kDa
Observed molecular weight 39-41 kDa
GenBank accession numberBC010004
Gene Symbol FDPS
Gene ID (NCBI) 2224
RRIDAB_2104749
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP14324
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FPPS (also known as FDPS) is a key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. The expression of FPPS was elevated in prostate tumor cell lines, indicating its potential role in prostate cancer progression.

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