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Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using FCRL5 Polyclonal antibody (26503-1-AP)

    WB analysis of Daudi using 26503-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 26503-1-AP (FCRL5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using FCRL5 Polyclonal antibody (26503-1-AP)

    WB analysis of Raji using 26503-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26503-1-AP (FCRL5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FCRL5 Polyclonal antibody

FCRL5 Polyclonal Antibody for WB, ELISA
Cat No. 26503-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BXMAS1, CD307, Fc receptor homolog 5, Fc receptor like 5, Fc receptor like protein 5, FcR like protein 5, FCRH5, FCRL5, IRTA2, PRO820

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDaudi cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26503-1-AP targets FCRL5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24351

Product name: Recombinant human FCRL5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 245-375 aa of BC101067

Sequence: FQITAMWSKDSGFYWCKAATMPYSVISDSPRSWIQVQIPASHPVLTLSPEKALNFEGTKVTLHCETQEDSLRTLYRFYHEGVPLRHKSVRCERGASISFSLTTENSGNYYCTADNGLGAKPSKAVSLSVTV

相同性解析による交差性が予測される生物種
Full Name Fc receptor-like 5
Calculated molecular weight 977 aa, 106 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC101067
Gene Symbol FCRL5
Gene ID (NCBI) 83416
RRIDAB_2880535
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FCRL5, also known as CD307 or IRTA2, is a surface protein expressed selectively on B cells and plasma cells (PMID: 11290337). This protein has both an immunoreceptor tyrosine-based activation motif (ITAM)-like sequence and two consensus immunoreceptor tyrosine-based inhibitory motifs (ITIM) in its cytoplasmic region (PMID: 17522256). FCRL5 is implicated in B cell development and lymphomagenesis (PMID: 11290337; 11453668). It may have an immunoregulatory role in marginal zone B-cells.

Protocols

Product Specific Protocols
WB protocol for FCRL5 antibody 26503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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