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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FCRL1 Polyclonal antibody (33184-1-AP)

    WB analysis using 33184-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33184-1-AP (FCRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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FCRL1 Polyclonal antibody

FCRL1 Polyclonal Antibody for WB, ELISA
Cat No. 33184-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CD307a, Fc receptor-like protein 1, FcRH1, FcR-like protein 1, IFGP family protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33184-1-AP targets FCRL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FCRL1 fusion protein Eg3713 相同性解析による交差性が予測される生物種
Full Name Fc receptor-like 1
Calculated molecular weight 429 aa, 47 kDa
Observed molecular weight47 kDa
GenBank accession numberBC033690
Gene Symbol FCRL1
Gene ID (NCBI) 115350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96LA6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fc receptor-like protein 1 (FCRL1) is a type I transmembrane glycoprotein that plays a crucial role in regulating B-cell receptor (BCR)-mediated signaling responses (PMID:15479727). It contains intracellular tyrosine-based motifs that can modulate BCR signaling, leading to changes in calcium flux, proliferation, and antibody production (PMID: 37822931). FCRL1 is involved in regulating B-cell development and activation, with its signaling pathways influencing ERK activation through interactions with proteins such as GRB2. This regulation is essential for maintaining B-cell homeostasis and preventing the development of autoreactive B cells (PMID: 34697226).

Protocols

Product Specific Protocols
WB protocol for FCRL1 antibody 33184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols