Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, Raji cells |
| Positive IHC detected in | human tonsillitis tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
21541-1-AP targets FCGR2B / CD32b in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16189 Product name: Recombinant human FCGR2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 254-310 aa of BC031992 Sequence: ALPGYPECREMGETLPEKPANPTNPDEADKVGAENTITYSLLMHPDALEEPDDQNRI 相同性解析による交差性が予測される生物種 |
| Full Name | Fc fragment of IgG, low affinity IIb, receptor (CD32) |
| Calculated molecular weight | 310 aa, 34 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC031992 |
| Gene Symbol | CD32b |
| Gene ID (NCBI) | 2213 |
| RRID | AB_2878881 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31994 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FCGR2B (also known as CD32b) is a transmembrane glycoprotein belonging to the immunoglobulin Fc receptor family. It functions primarily as a low-affinity inhibitory receptor for IgG antibodies. Through its intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM), it mediates negative signaling, suppressing overactivation in immune cells (such as B cells, monocytes/macrophages.), regulating antibody production, and maintaining immune homeostasis. It is also involved in clearing immune complexes and modulating the threshold for autoimmune responses.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FCGR2B / CD32b antibody 21541-1-AP | Download protocol |
| WB protocol for FCGR2B / CD32b antibody 21541-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Ther High-dose systemic adeno-associated virus vector administration causes liver and sinusoidal endothelial cell injury | ||
Curr Issues Mol Biol Identification and Validation of a Macrophage Phagocytosis-Related Gene Signature for Prognostic Prediction in Colorectal Cancer (CRC). |