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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FCER1G Recombinant antibody (85390-2-RR)

    WB analysis using 85390-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85390-2-RR (FCER1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85390-2-RR

    Affinity and Kinetic Characterization of 85390-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85390-2-RR against Human FCER1G were performed. The affinity constant is 1.16 nM.

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FCER1G Recombinant antibody

FCER1G Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85390-2-RR
Clone No.242829E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, ELISA

Synonyms

242829E3, Fc epsilon RI gamma, Fc receptor gamma-chain, Fc-epsilon RI-gamma, FceRI gamma

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  100 μL, 1000 μg/ml
20 μL, 1000 μg/ml
100 μL, 1000 μg/ml

¥85,000/ 100 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, RAW 264.7 cells, pig spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85390-2-RR targets FCER1G in WB, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FCER1G fusion protein Ag4442 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
Calculated molecular weight 87 aa, 10 kDa
Observed molecular weight10 kDa
GenBank accession numberBC033872
Gene Symbol FcRγ
Gene ID (NCBI) 2207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FCER1G (Fc receptor gamma-chain, FcRgamma), also known as Fc-epsilon RI-gamma, or IgE Fc receptor subunit gamma (FceRI gamma), is a component of the high-affinity immunoglobulin E (IgE) receptor (Fc epsilon RI) which is a tetrameric complex of one alpha subunit, one beta subunit, and two disulfide-linked gamma subunits (PMID: 2146219; 2531187). Fc epsilon RI is present exclusively on mast cells and basophils, mediating allergic inflammatory signaling (PMID: 17438574). FCER1G is widely expressed and contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors (PMID: 19098920).

Protocols

Product Specific Protocols
WB protocol for FCER1G antibody 85390-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols