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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using FCER1G Recombinant antibody (85390-1-RR)

    IF Staining of THP-1 using 85390-1-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCER1G antibody (85390-1-RR, Clone: 242829C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using FCER1G Recombinant antibody (85390-1-RR)

    IF Staining of RAW 264.7 using 85390-1-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using FCER1G antibody (85390-1-RR, Clone: 242829C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85390-1-RR

    Affinity and Kinetic Characterization of 85390-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85390-1-RR against Human FCER1G were performed. The affinity constant is 2.23 nM.

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FCER1G Recombinant antibody

FCER1G Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 85390-1-RR
Clone No.242829C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, ELISA

Synonyms

FCRG, FceRI gamma, Fc-epsilon RI-gamma, Fc receptor gamma-chain, Fc epsilon RI gamma

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inTHP-1 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85390-1-RR targets FCER1G in IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FCER1G fusion protein Ag4442 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
Calculated molecular weight 87 aa, 10 kDa
GenBank accession numberBC033872
Gene Symbol FcRγ
Gene ID (NCBI) 2207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FCER1G (Fc receptor gamma-chain, FcRgamma), also known as Fc-epsilon RI-gamma, or IgE Fc receptor subunit gamma (FceRI gamma), is a component of the high-affinity immunoglobulin E (IgE) receptor (Fc epsilon RI) which is a tetrameric complex of one alpha subunit, one beta subunit, and two disulfide-linked gamma subunits (PMID: 2146219; 2531187). Fc epsilon RI is present exclusively on mast cells and basophils, mediating allergic inflammatory signaling (PMID: 17438574). FCER1G is widely expressed and contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors (PMID: 19098920).

Protocols

Product Specific Protocols
IF protocol for FCER1G antibody 85390-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols