Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cell, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33578-1-AP targets FBXW8 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36986 Product name: Recombinant human FBXW8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC037296 Sequence: MDDYSLDEFRRRWQEELAQAQAPKKRRRPEAAERRARRPEVGSGRSEQASGDPALAQGLLEGAGRPPAARATRAEGQDVASRSRSPLAREGAGGGEQLVDQLIRDLNEMNDVPFFDIQLPYELAINIFQYLDRKELGRCAQVSKTWKVIAEDEVLWYRLCQQEGHLPDSSISDYSCWKLIFQECRAKEHMLQTNWKNRKGAVSELEHVPDTVLCDVHSHDGVVIAGYTSGDVRVWDTRTWDYVAPFLESEDEEDEPGMQPNVSFVRINSSLAVAAYEDGFLNIWDLRTGKYPVHRFEHDARIQ 相同性解析による交差性が予測される生物種 |
| Full Name | F-box and WD repeat domain containing 8 |
| Calculated molecular weight | 67 kDa |
| Observed molecular weight | 67 kDa, 60 kDa |
| GenBank accession number | BC037296 |
| Gene Symbol | FBXW8 |
| Gene ID (NCBI) | 26259 |
| RRID | AB_3743018 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N3Y1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fbxw8 (also known as Fbw6 or Fbx29) is an F-box protein that is assembled with Cul7 in an SCF-like E3 complex. FBXW8 directs the proteasomal degradation of β-TrCP1 in MAPK pathway-dependent manner, and the attenuation of β-TrCP1 by FBXW8 is important for Cdc25A-mediated cell cycle transition from G1 phase to S phase as well as DNA damage-free progression of S phase (PMID: 33742524).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXW8 antibody 33578-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |