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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of HEK-293 using 10872-1-AP (same clone as 10872-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of HeLa using 10872-1-AP (same clone as 10872-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of HepG2 using 10872-1-AP (same clone as 10872-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of K-562 using 10872-1-AP (same clone as 10872-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human ovary tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of human ovary using 10872-1-AP (same clone as 10872-1-PBS)

    human ovary tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of mouse kidney using 10872-1-AP (same clone as 10872-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    WB analysis of rat liver using 10872-1-AP (same clone as 10872-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 10872-1-AP (FBXO5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human placenta using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human tonsillitis using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FBXO5 Polyclonal antibody (10872-1-AP)

    IHC staining of human tonsillitis using 10872-1-AP (same clone as 10872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10872-1-AP (FBXO5 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10872-1-PBS in a different storage buffer formulation.

FBXO5 Polyclonal antibody, PBS Only

FBXO5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10872-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10872-1-PBS targets FBXO5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1296

Product name: Recombinant human FBXO5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-447 aa of BC018905

Sequence: MSRRPCSCALRPPRCSCSASPSAVTAAGRPRPSDSCKEESSTLSVKMKCDFNCNHVHSGLKLVKPDDIGRLVSYTPAYLEGSCKDCIKDYERLSCIGSPIVSPRIVELETESKRLHNKENQHVQQTLNSTNEIEALETSRLYEDSGYSSFSLQSGLSEHEEGSLLEENFGDSLQSCLLQIQSPDQYPNKNLLPVLHFEKVVCSTLKKNAKRNPKVDREMLKEIIARGNFRLQNIIGRKMGLECVDILSELFRRGLRHVLATILAQLSDMDLINVSKVSTTWKKILEDDKGAFQLYSKAIQRVTENNNKFSPHASTREYVMFRTPLASVQKSAAQTSLKKDAQTKLSNQGDQKGSTYSRHNEFSEVAKTLKKNESLKACIRCNSPAKYDCYLQRATCKREGCGFDYCTKCLCNYHTTKDCSDGKLLKASCKIGPLPGTKKSKKNLRRL

相同性解析による交差性が予測される生物種
Full Name F-box protein 5
Calculated molecular weight 54 kDa
Observed molecular weight 56 kDa, 45 kDa
GenBank accession numberBC018905
Gene Symbol FBXO5
Gene ID (NCBI) 26271
RRIDAB_2247034
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKT4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.

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