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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO32 Monoclonal antibody (67172-1-Ig)

    WB analysis using 67172-1-Ig (same clone as 67172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    WB analysis of human heart using 67172-1-Ig (same clone as 67172-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    WB analysis of pig heart using 67172-1-Ig (same clone as 67172-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    WB analysis of pig skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67172-1-Ig (FBXO32 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IHC staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IHC staining of rat skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IHC staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IHC staining of rat skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67172-1-Ig (FBXO32 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IF Staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using FBXO32 Monoclonal antibody (67172-1-Ig)

    IF Staining of mouse skeletal muscle using 67172-1-Ig (same clone as 67172-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBXO32 antibody (67172-1-Ig, Clone: 3C2G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67172-1-PBS in a different storage buffer formulation.

FBXO32 Monoclonal antibody, PBS Only

FBXO32 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67172-1-PBS
Clone No.3C2G3

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

3C2G3, Atrogin 1, Atrogin-1, F box only protein 32, F box protein 32

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67172-1-PBS targets FBXO32 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25247

Product name: Recombinant human FBXO32 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-216 aa of BC024030

Sequence: IAQKNFMNILEKVVLKVLEDQQNIRLIRELLQTLYTSLCTLVQRVGKSVLVGNINMWVYRMETILHWQQQLNNIQITRPAFKGLTFTDLPLCLQLNIMQRLSDGRDLVSLGQAAPDLHVLSEDRLLWKKLCQYHFSERQIRKRLILSDKGQLDWKKMYFKLVRCYPRKEQYGDTLQLRKHCHILSWKGTDHPCTANNPESCSVSLSPQDFINLFKF

相同性解析による交差性が予測される生物種
Full Name F-box protein 32
Calculated molecular weight 355 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC024030
Gene Symbol FBXO32
Gene ID (NCBI) 114907
RRIDAB_2882468
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ969P5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.

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