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  • KD/KO Validated

FBXO27 Polyclonal antibody

FBXO27 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA, IF

Conjugate

Unconjugated

Cat no : 14570-1-AP

Synonyms

FBX27, F-box/G-domain protein 5, F-box only protein 27, FBG5


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Tested Applications

Positive WB detected inPANC-1 cells
Positive IP detected inPANC-1 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14570-1-AP targets FBXO27 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO27 fusion protein Ag6157 相同性解析による交差性が予測される生物種
Full Name F-box protein 27
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC030060
Gene symbol FBXO27
Gene ID (NCBI) 126433
RRIDAB_2104422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO27 is a glycoprotein-specific F-box protein that is part of the SCF (SKP1/CUL1/F-box protein) ubiquitin ligase complex, and SCFFBXO27 ubiquitinates glycoproteins in damaged lysosomes will regulate autophagic machinery recruitment (PMID: 28743755).Unlike F-box proteins in other SCF complexes, FBXO27 is subject to N-myristoylation, which localizes it to membranes, allowing it to accumulate rapidly around damaged lysosomes.

Protocols

Product Specific Protocols
IHC protocol for FBXO27 antibody 14570-1-APDownload protocol
IP protocol for FBXO27 antibody 14570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Proc Natl Acad Sci U S A

Ubiquitination of exposed glycoproteins by SCF(FBXO27) directs damaged lysosomes for autophagy.

Authors - Yukiko Yoshida
  • KO Validated
humanWB,IF

Autophagy

TRIM16-mediated lysophagy suppresses high-glucose-accumulated neuronal Aβ

Authors - Chang Woo Chae