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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO16 Polyclonal antibody (32063-1-AP)

    WB analysis using 32063-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32063-1-AP (FBXO16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of T-47D cells using FBXO16 Polyclonal antibody (32063-1-AP)

    IP experiment of T-47D using 32063-1-AP

    IP result of anti-FBXO16 (IP:32063-1-AP, 4ug; Detection:32063-1-AP 1:1000) with T-47D cells lysate 1120 ug.

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FBXO16 Polyclonal antibody

FBXO16 Polyclonal Antibody for WB, IP, ELISA
Cat No. 32063-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

F box only protein 16, F box protein 16, FBX16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, T-47D cells
Positive IP detected inT-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32063-1-AP targets FBXO16 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO16 fusion protein Ag36230 相同性解析による交差性が予測される生物種
Full Name F-box protein 16
Calculated molecular weight 292 aa, 35 kDa
Observed molecular weight35 kDa
GenBank accession numberBC074986
Gene Symbol FBXO16
Gene ID (NCBI) 157574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8IX29
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO16 is a member of the F-box protein family, which functions as a substrate recognition subunit of the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex. High expression levels of FBXO16 are associated with better prognosis in cancer patients. For example, in ovarian cancer, patients with higher FBXO16 expression tend to have a more favorable outcome (PMID: 34333526). This suggests that FBXO16 could serve as a potential biomarker for cancer prognosis and therapeutic targeting.

Protocols

Product Specific Protocols
WB protocol for FBXO16 antibody 32063-1-APDownload protocol
IP protocol for FBXO16 antibody 32063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols