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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using FBXL13 Polyclonal antibody (17139-1-AP)

    IHC staining of human liver using 17139-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17139-1-AP (FBXL13 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using FBXL13 Polyclonal antibody (17139-1-AP)

    IHC staining of human liver using 17139-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17139-1-AP (FBXL13 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FBXL13 Polyclonal antibody (17139-1-AP)

    IF Staining of HeLa using 17139-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17139-1-AP (FBXL13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

FBXL13 Polyclonal antibody

FBXL13 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 17139-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

DRC6, Dynein regulatory complex subunit 6, FBL13, F-box and leucine-rich repeat protein 13, F-box/LRR-repeat protein 13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17139-1-AP targets FBXL13 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10903

Product name: Recombinant human FBXL13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 358-707 aa of BC031285

Sequence: NDMPTLTDNCVKALVEKCSRITSLVFTGAPHISDCTFRALSACKLRKIRFEGNKRVTDASFKFIDKNYPNLSHIYMADCKGITDSSLRSLSPLKQLTVLNLANCVRIGDMGLKQFLDGPASMRIRELNLSNCVRLSDASVMKLSERCPNLNYLSLRNCEHLTAQGIGYIVNIFSLVSIDLSGTDISNEAFCKSSLILEHLDVSYCSQLSDMIIKALAIYCINLTSLSIAGCPKITDSAMEMLSAKCHYLHILDISGCVLLTDQILEDLQIGCKQLRILKMQYCTNISKKAAQRMSSKVQQQEYNTNGPPRWFGYDREGNPVTELDNITSSKGALELTVKKSTYSSEDQAA

相同性解析による交差性が予測される生物種
Full Name F-box and leucine-rich repeat protein 13
Calculated molecular weight 707 aa, 81 kDa
GenBank accession numberBC031285
Gene Symbol FBXL13
Gene ID (NCBI) 222235
RRIDAB_2103869
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEE6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for FBXL13 antibody 17139-1-APDownload protocol
IF protocol for FBXL13 antibody 17139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols