Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells, A431 cells, NIH/3T3 cells, mouse lung tissue, mouse thymus tissue, mouse spleen tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87549-1-RR targets FBX4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5013 Product name: Recombinant human FBX4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-387 aa of BC048098 Sequence: MAGSEPRSGTNSPPPPFSDWGRLEAAILSGWKTFWQSVSKERVARTTSREEVDEAASTLTRLPIDVQLYILSFLSPHDLCQLGSTNHYWNETVRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPISEVTDGAFFDYMAVYRMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVNFQLNNQHKFNILILYSTTRKERDRAREEHTSAVNKMFSRHNEGDDQQGSRYSVIPQIQKVCEVVDGFIYVANAEAHKRHEWQDEFSHIMAMTDPAFGSSGRPLLVLSCISQGDVKRMPCFYLAHELHLNLLNHPWLVQDTEAETLTGFLNGIEWILEEVESKRAR 相同性解析による交差性が予測される生物種 |
| Full Name | F-box protein 4 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC048098 |
| Gene Symbol | FBXO4/FBX4 |
| Gene ID (NCBI) | 26272 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UKT5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBX4 (F-Box Protein 4), also known as FBXO4, is a member of the F-box protein family. FBX4 is involved in the ubiquitination of various target proteins, leading to their degradation. It has been shown to regulate the cell cycle by targeting proteins such as cyclin D1 for ubiquitination and degradation. Mutations in FBX4 have been linked to various diseases, including spermatogenic failure and esophageal cancer. In cancer, FBX4 has been shown to act as a tumor suppressor. Loss of FBX4 function can lead to increased cell proliferation and tumor development. For example, FBX4 knockout mice have been shown to develop various tumors, including lymphoblastic lymphoma and mammary carcinoma. FBX4 has two isforms: 44 kDa and 35 kDa. The observd molecular weight is consistent with the result in PMID: 21242966.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FBX4 antibody 87549-1-RR | Download protocol |
| WB protocol for FBX4 antibody 87549-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |