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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Asprosin Polyclonal antibody (20251-1-AP)

    WB analysis using 20251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20251-1-AP (Asprosin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using FBN1-Specific Polyclonal antibody (20251-1-AP)

    IHC staining of human ovary using 20251-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 20251-1-AP (FBN1-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using FBN1-Specific Polyclonal antibody (20251-1-AP)

    IHC staining of human ovary using 20251-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 20251-1-AP (FBN1-Specific Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using FBN1-Specific Polyclonal antibody (20251-1-AP)

    IHC staining of human skin cancer using 20251-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20251-1-AP (FBN1-Specific Antibody) at dilution of 1:50 (under 40x lens).

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Asprosin Polyclonal antibody

Asprosin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20251-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

FBN1-Specific, FBN1, Fibrillin 1 , FBN, Fibrillin-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA172 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20251-1-AP targets Asprosin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name fibrillin 1
Calculated molecular weight 30 kDa
Observed molecular weight32 kDa
GenBank accession numberNM_000138
Gene Symbol Fibrillin 1
Gene ID (NCBI) 2200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35555
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Asprosin is a hormone derived from fibrillin-1 that plays a role in regulating glucose metabolism and appetite. It's released by adipose tissue during fasting and stimulates the liver to release glucose into the bloodstream, ensuring energy availability. Asprosin also influences appetite and body weight by acting on neurons in the hypothalamus. It has been linked to various metabolic diseases, including obesity, type 2 diabetes, and polycystic ovary syndrome (PCOS). The detected molecular size is consistent with that in the article PMID: 35079041.

Protocols

Product Specific Protocols
WB protocol for Asprosin antibody 20251-1-APDownload protocol
IHC protocol for Asprosin antibody 20251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols