• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBL Monoclonal antibody, PBS Only

FBL Monoclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A9E8

Cat no : 66985-1-PBS

Synonyms

FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1

Validation Data Gallery

Applications:
  • WB analysis using 66985-1-Ig (same clone as 66985-1-PBS)

    WB analysis using 66985-1-Ig (same clone as 66985-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66985-1-Ig (FBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • WB analysis using 66985-1-Ig (same clone as 66985-1-PBS)

    WB analysis using 66985-1-Ig (same clone as 66985-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66985-1-Ig (FBL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66985-1-Ig (same clone as 66985-1-PBS)

    IP experiment of HeLa using 66985-1-Ig (same clone as 66985-1-PBS)

    IP result of anti-FBL (IP:66985-1-Ig, 5ug; Detection:66985-1-Ig 1:1000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66985-1-Ig (same clone as 66985-1-PBS)

    IHC staining of human liver cancer using 66985-1-Ig (same clone as 66985-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66985-1-Ig (FBL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBL antibody included
  • IHC staining of human liver cancer using 66985-1-Ig (same clone as 66985-1-PBS)

    IHC staining of human liver cancer using 66985-1-Ig (same clone as 66985-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66985-1-Ig (FBL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBL antibody included
  • IF Staining of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

    IF Staining of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66985-1-Ig (same clone as 66985-1-PBS)

    IF Staining of HeLa using 66985-1-Ig (same clone as 66985-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

    IF Staining of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBL antibody (66985-1-Ig, Clone: 3A9E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

    FC experiment of HepG2 using 66985-1-Ig (same clone as 66985-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FBL (66985-1-Ig, Clone:3A9E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66985-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy FBL Ready-To-Use IHC Kit. FBL primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66985-1-PBS targets FBL in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FBL fusion protein Ag8841 相同性解析による交差性が予測される生物種
Full Name fibrillarin
Calculated molecular weight 321 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC019260
Gene symbol FBL
Gene ID (NCBI) 2091
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).