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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FATS Polyclonal antibody (30190-1-AP)

    WB analysis using 30190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30190-1-AP (FATS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FATS Polyclonal antibody (30190-1-AP)

    IF Staining of A431 using 30190-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FATS antibody (30190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

FATS Polyclonal antibody

FATS Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30190-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

bA422P15.2, C10orf90, FATS, FLJ32938

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, mouse liver tissue, rat brain tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30190-1-AP targets FATS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FATS fusion protein Ag32956 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 90
Calculated molecular weight78 kDa
Observed molecular weight 67-77 kDa
GenBank accession numberNM_001004298.2
Gene Symbol FATS/C10orf90
Gene ID (NCBI) 118611
RRIDAB_2935528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M02
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FATS, also known as centrosomal protein C10orf90, bA422P15.2, belongs to a novel class of enzymes that acts as an E2-E3 hybrid enzyme for assembly of polyubiquitin chains. FATS is an E2-independent ubiquitin ligase that stabilizes p53 and promotes its activation in response to DNA damage (PMID: 24240685). FATS acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53.

Protocols

Product Specific Protocols
WB protocol for FATS antibody 30190-1-APDownload protocol
IF protocol for FATS antibody 30190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols