Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HeLa |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | transfected cells, HeLa cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
Product Information
18392-1-AP targets FASTKD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FASTKD3 fusion protein Ag13083 相同性解析による交差性が予測される生物種 |
Full Name | FAST kinase domains 3 |
Calculated molecular weight | 76 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC001295 |
Gene Symbol | FASTKD3 |
Gene ID (NCBI) | 79072 |
RRID | AB_2878541 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14CZ7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAST kinase domain-containing protein 3(FASTKD3) belongs to FASTKD family.FASTKD3 is an essential component of mitochondrial respiration that may modulate energy balance in cells exposed to adverse conditions by functionally coupling mitochondrial protein synthesis to respiration. The expression of endogenous FASTKD3 is very weak. This antibody detects the c-terminal of FASTKD3.
Protocols
Product Specific Protocols | |
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WB protocol for FASTKD3 antibody 18392-1-AP | Download protocol |
IHC protocol for FASTKD3 antibody 18392-1-AP | Download protocol |
IF protocol for FASTKD3 antibody 18392-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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J Biol Chem Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.
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