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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FASN Recombinant antibody

FASN Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1L9

Cat no : 81079-1-RR

Synonyms

FAS, FASN, fatty acid synthase, OA 519, SDR27X1

Validation Data Gallery

Applications:
  • WB analysis using 81079-1-RR

    WB analysis using 81079-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81079-1-RR (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 81079-1-RR

    IF Staining of HeLa using 81079-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FASN antibody (81079-1-RR, Clone: 1L9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, U-251 cells, Neuro-2a cells, HSC-T6 cells, mouse liver tissue, rat liver tissue
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

81079-1-RR targets FASN in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FASN fusion protein Ag0975 相同性解析による交差性が予測される生物種
Full Name fatty acid synthase
Calculated molecular weight 272 kDa
Observed molecular weight250-270 kDa
GenBank accession numberBC007909
Gene symbol FASN
Gene ID (NCBI) 2194
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

Protocols

Product Specific Protocols
WB protocol for FASN antibody 81079-1-RRDownload protocol
IF protocol for FASN antibody 81079-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols