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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FASN Monoclonal antibody (66591-1-Ig)

    WB analysis of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    WB result of FASN antibody (66591-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FASN Monoclonal antibody (66591-1-Ig)

    WB analysis of HepG2 using 66591-1-Ig (same clone as 66591-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FASN Monoclonal antibody (66591-1-Ig)

    WB analysis of HEK-293 using 66591-1-Ig (same clone as 66591-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using FASN Monoclonal antibody (66591-1-Ig)

    WB analysis of Jurkat using 66591-1-Ig (same clone as 66591-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FASN Monoclonal antibody (66591-1-Ig)

    IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66591-1-Ig (FASN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FASN Monoclonal antibody (66591-1-Ig)

    IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66591-1-Ig (FASN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FASN Monoclonal antibody (66591-1-Ig)

    IF Staining of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (66591-1-Ig, Clone: 1B6E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FASN Monoclonal antibody, PBS Only

FASN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66591-1-PBS
Clone No.1B6E4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Type I fatty acid synthase, fatty acid synthase, FAS, 1B6E4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66591-1-PBS targets FASN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0975

Product name: Recombinant human FASN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 142-439 aa of BC007909

Sequence: QTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

相同性解析による交差性が予測される生物種
Full Name fatty acid synthase
Calculated molecular weight 272 kDa
Observed molecular weight 272 kDa
GenBank accession numberBC007909
Gene Symbol FASN
Gene ID (NCBI) 2194
RRIDAB_2881951
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP49327
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

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