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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FAS/CD95 Polyclonal antibody

FAS/CD95 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13098

Synonyms

ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS1, FASLG receptor, FASTM, TNFRSF6

Validation Data Gallery

Applications:
  • FC experiment of Jurkat using CL488-13098

    FC experiment of Jurkat using CL488-13098

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FAS/CD95 (CL488-13098) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 13098-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-13098 targets FAS/CD95 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAS/CD95 fusion protein Ag3718 相同性解析による交差性が予測される生物種
Full Name Fas (TNF receptor superfamily, member 6)
Calculated molecular weight 35-38 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC012479
Gene symbol FAS
Gene ID (NCBI) 355
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 FAS/CD95 antibody CL488-13098Download protocol
Standard Protocols
Click here to view our Standard Protocols