Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 18121-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 18121-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 18121-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 18121-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 18121-1-AP (FARSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 18121-1-AP
IP result of anti-FARSA (IP:18121-1-AP, 4ug; Detection:18121-1-AP 1:2000) with MCF-7 cells lysate 3240 ug.
IHC staining of human liver cancer using 18121-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18121-1-AP (FARSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 18121-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18121-1-AP (FARSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18121-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 18121-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18121-1-AP (FARSA antibody) at dilution of 1:100 (under 40x lens).
IF Staining of MCF-7 using 18121-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FARSA antibody (18121-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of A431 using 18121-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FARSA antibody (18121-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HeLa using 18121-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FARSA antibody (18121-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

FARSA Polyclonal antibody

FARSA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18121-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

Phenylalanine--tRNA ligase alpha subunit, FARSLA, FARSL, FARS, EC:6.1.1.20

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, K-562 cells, MCF-7 cells, HepG2 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human liver cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, HeLa cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:4000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IF	See 2 publications below
IP	See 1 publications below
ELISA	See 1 publications below
RIP	See 1 publications below

Product Information

18121-1-AP targets FARSA in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12647 Product name: Recombinant human FARSA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-300 aa of BC006495 Sequence: MADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETELSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTH 相同性解析による交差性が予測される生物種
Full Name	phenylalanyl-tRNA synthetase, alpha subunit
Calculated molecular weight	58 kDa
Observed molecular weight	57 kDa
GenBank accession number	BC006495
Gene Symbol	FARSA
Gene ID (NCBI)	2193
RRID	AB_2102509
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y285
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for FARSA antibody 18121-1-AP	Download protocol
IHC protocol for FARSA antibody 18121-1-AP	Download protocol
IP protocol for FARSA antibody 18121-1-AP	Download protocol
WB protocol for FARSA antibody 18121-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,rat	WB	Nat Commun Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Authors - Megha Jhanji View Article
human	WB	Clin Genet FARS1-related disorders caused by bi-allelic mutations in cytosolic phenylalanyl-tRNA synthetase genes: Look beyond the lungs! Authors - Luise A Schuch View Article
human,rat,mouse	IF,ELISA	Nat Commun Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ. Authors - Qian Zhou KO Validated View Article
human	WB,IP	Autoimmun Rev Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase Authors - Yoshinao Muro View Article
human,mouse	WB,IF,RIP	Nat Commun Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA Authors - Mirjana Malnar Črnigoj View Article
Human	WB	Clin Exp Rheumatol Anti-Zo antibodies in Japanese myositis patients detected by a newly developed ELISA Authors - Yoshinao Muro View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18121-1-AP

WB analysis using 18121-1-AP

WB analysis of K-562 using 18121-1-AP

WB analysis of MCF-7 using 18121-1-AP

IP experiment of MCF-7 using 18121-1-AP

IHC staining of human liver cancer using 18121-1-AP

IHC staining of human liver cancer using 18121-1-AP

IHC staining of human kidney using 18121-1-AP

IHC staining of human kidney using 18121-1-AP

IF Staining of MCF-7 using 18121-1-AP

IF Staining of A431 using 18121-1-AP

IF Staining of HeLa using 18121-1-AP

FARSA Polyclonal antibody

FARSA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

FARS1-related disorders caused by bi-allelic mutations in cytosolic phenylalanyl-tRNA synthetase genes: Look beyond the lungs!

Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.

Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Anti-Zo antibodies in Japanese myositis patients detected by a newly developed ELISA