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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FARP1 Polyclonal antibody (24014-1-AP)

    WB analysis using 24014-1-AP (same clone as 24014-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24014-1-AP (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24014-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FARP1 Polyclonal antibody (24014-1-AP)

    WB analysis using 24014-1-AP (same clone as 24014-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24014-1-AP (FARP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24014-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using FARP1 Polyclonal antibody (24014-1-AP)

    IP experiment of HeLa using 24014-1-AP (same clone as 24014-1-PBS)

    IP result of anti-FARP1 (IP:24014-1-AP, 4ug; Detection:24014-1-AP 1:1000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 24014-1-PBS in a different storage buffer formulation.

FARP1 Polyclonal antibody, PBS Only

FARP1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 24014-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

Pleckstrin homology domain-containing family C member 2, PH domain-containing family C member 2, FERM, RhoGEF and pleckstrin domain-containing protein 1, FERM, ARHGEF and pleckstrin domain-containing protein 1, Chondrocyte-derived ezrin-like protein

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24014-1-PBS targets FARP1 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21225

Product name: Recombinant human FARP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 347-539 aa of BC071592

Sequence: VLDYVKEGGHKKVQFERKHSKIHSIRSLASQPTELNSEVLEQSQQSTSLTFGEGAESPGGQSCRRGKEPKVSAGEPGSHPSPAPRRSPAGNKQADGAASAPTEEEEEVVKDRTQQSKPQPPQPSTGSLTGSPHLSELSVNSQGGVAPANVTLSPNLSPDTKQASPLISPLLNDQACPRTDDEDEGRRKRFPTD

相同性解析による交差性が予測される生物種
Full Name FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
Calculated molecular weight 1076 aa, 122 kDa
Observed molecular weight122 kDa
GenBank accession numberBC071592
Gene Symbol FARP1
Gene ID (NCBI) 10160
RRIDAB_3085720
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9Y4F1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FARP1 is a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth.

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