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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FANK1 Polyclonal antibody (17167-1-AP)

    WB analysis using 17167-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17167-1-AP (FANK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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FANK1 Polyclonal antibody

FANK1 Polyclonal Antibody for WB, ELISA
Cat No. 17167-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

UNQ6504/PRO21382, HSD13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17167-1-AP targets FANK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FANK1 fusion protein Ag10639 相同性解析による交差性が予測される生物種
Full Name fibronectin type III and ankyrin repeat domains 1
Calculated molecular weight 345 aa, 38 kDa
Observed molecular weight38-40 kDa
GenBank accession numberBC024189
Gene Symbol FANK1
Gene ID (NCBI) 92565
RRIDAB_3669264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TC84
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FANK1 (Fibronectin type 3 and ankyrin repeat domains protein 1), also known as HSD13. It is expected to be located in the nucleus. And mostly restricted to testis (PMID: 17604233). Fibronectin type III and ankyrin repeat domains 1(Fank1) is a nuclear protein expressed during the transition from the meiotic phase to the haploid phase of spermatogenesis. While little is known about the function of Fank1 during spermatogenesis, gene ontology analysis suggests that it may be a transcription factor. H. Wang explored the role of Fank1 using the yeast two-hybrid system to screen for cellular proteins that may interact with Fank1. In this screen, they identified the Jun activation domain-binding protein 1 (Jab1) as a potential Fank1-interacting protein (PMID: 20978819).

Protocols

Product Specific Protocols
WB protocol for FANK1 antibody 17167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols