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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FANCM Recombinant antibody

FANCM Recombinant Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230233G5

Cat no : 82897-2-RR

Synonyms

FAAP250, FANCM, Fanconi anemia group M protein, KIAA1596, Protein FACM, Protein Hef ortholog

Validation Data Gallery

Applications:
  • IHC staining of mouse testis using 82897-2-RR

    IHC staining of mouse testis using 82897-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 82897-2-RR

    IHC staining of mouse testis using 82897-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 82897-2-RR

    IF Staining of HeLa using 82897-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCM antibody (82897-2-RR, Clone: 230233G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

82897-2-RR targets FANCM in IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FANCM fusion protein Ag34447 相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group M
GenBank accession numberBC140776
Gene symbol FANCM
Gene ID (NCBI) 57697
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for FANCM antibody 82897-2-RRDownload protocol
IF protocol for FANCM antibody 82897-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols