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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FANCM Recombinant antibody (82897-1-RR)

    IF Staining of HeLa using 82897-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCM antibody (82897-1-RR, Clone: 230233A12 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82897-1-RR

    Affinity and Kinetic Characterization of 82897-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82897-1-RR against Human FANCM were performed. The affinity constant is below 1 pM.

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FANCM Recombinant antibody

FANCM Recombinant Antibody for IF/ICC, ELISA
Cat No. 82897-1-RR
Clone No.230233A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82897-1-RR targets FANCM in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FANCM fusion protein Ag34447 相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group M
GenBank accession numberBC140776
Gene Symbol FANCM
Gene ID (NCBI) 57697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IYD8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for FANCM antibody 82897-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols