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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FANCD2 Recombinant monoclonal antibody (83946-4-RR)

    WB analysis using 83946-4-RR (same clone as 83946-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83946-4-RR (FANCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83946-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FANCD2 Recombinant monoclonal antibody (83946-4-RR)

    WB analysis of HeLa using 83946-4-RR (same clone as 83946-4-PBS)

    WB result of FANCD2 antibody (83946-4-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FANCD2 transfected HeLa cells. This data was developed using the same antibody clone with 83946-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FANCD2 Recombinant monoclonal antibody (83946-4-RR)

    IF Staining of HeLa using 83946-4-RR (same clone as 83946-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCD2 antibody (83946-4-RR, Clone: 241048G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83946-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using FANCD2 Recombinant monoclonal antibody (83946-4-RR)

    FC experiment of HeLa using 83946-4-RR (same clone as 83946-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FANCD2 Recombinant antibody (83946-4-RR, Clone:241048G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83946-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83946-4-RR

    Affinity and Kinetic Characterization of 83946-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83946-4-RR against Human FANCD2 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

FANCD2 Recombinant monoclonal antibody, PBS Only

FANCD2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83946-4-PBS
Clone No.241048G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241048G8, FA4, FACD, FAD, FAD2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83946-4-PBS targets FANCD2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag29460

Product name: Recombinant human FANCD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-127 aa of NM_001018115

Sequence: MVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLLKISGIILKTGESQNQLAVDQIAFQKKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDEEASM

相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group D2
Calculated molecular weight 164 kDa
Observed molecular weight150-160 kDa
GenBank accession numberNM_001018115
Gene Symbol FANCD2
Gene ID (NCBI) 2177
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9BXW9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Amongst the FA genes, Fanconi Anemia Group D2 Protein (FANCD2) is the most evolutionarily conserved gene, demonstrating a high sequence similarity from lower eukaryotes to humans. Upon activation, Fanconi anemia group D2 protein (FANCD2) is monoubiquitinated at K561 and works in cooperation with other FA and non-FA proteins to repair DNA damage. In regards to the FA pathway, monoubiquitinated or activated FANCD2 is often portrayed as the functional representative for the functions of activated FA signaling. (PMID: 28825622)

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