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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FANCA Polyclonal antibody (11975-1-AP)

    WB analysis of HeLa cells using 11975-1-AP (same clone as 11975-1-PBS)

    WB results of FANCA (11975-1-AP, 1:500) with si-control and si-FANCA transfected HeLa cells. This data was developed using the same antibody clone with 11975-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FANCA Polyclonal antibody (11975-1-AP)

    WB analysis using 11975-1-AP (same clone as 11975-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11975-1-AP (FANCA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FANCA Polyclonal antibody (11975-1-AP)

    IHC staining of human colon cancer using 11975-1-AP (same clone as 11975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FANCA Polyclonal antibody (11975-1-AP)

    IHC staining of human colon cancer using 11975-1-AP (same clone as 11975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FANCA Polyclonal antibody (11975-1-AP)

    IHC staining of human stomach cancer using 11975-1-AP (same clone as 11975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using FANCA Polyclonal antibody (11975-1-AP)

    IHC staining of human cervical cancer using 11975-1-AP (same clone as 11975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse intestine using FANCA Polyclonal antibody (11975-1-AP)

    IHC staining of mouse intestine using 11975-1-AP (same clone as 11975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using FANCA Polyclonal antibody (11975-1-AP)

    IHC staining of mouse thymus using 11975-1-AP (same clone as 11975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using FANCA Polyclonal antibody (11975-1-AP)

    IHC staining of rat small intestine using 11975-1-AP (same clone as 11975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using FANCA Polyclonal antibody (11975-1-AP)

    IHC staining of rat testis using 11975-1-AP (same clone as 11975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11975-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FANCA Polyclonal antibody, PBS Only

FANCA Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11975-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

FANCH, FAH, FACA, FAA, FA1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11975-1-PBS targets FANCA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2598

Product name: Recombinant human FANCA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-297 aa of BC008979

Sequence: MSDSWVPNSASGQDPGGRRRAWAELLAGRVKREKYNPERAQKLKESAVRLLRSHQDLNALLLEVEGPLCKKLSLSKVIDCDSSEAYANHSSSFIGSALQDQASRLGVPVGILSAGMVASSVGQICTAPAETSHPVLLTVEQRKKLSSLLEFAQYLLAHSMFSRLSFCQELWKIQSSLLLEAVWHLHVQGIVSLQELLESHPDMHAVGSWLFRNLCCLCEQMEASCQHADVARAMLSDFVQMFVLRGFQKNSDLRRTVEPEKMPQVAVDVLQRMLIFALDALAAGVQEESSTHKIVRC

相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group A
Calculated molecular weight 163 kDa
Observed molecular weight 163 kDa
GenBank accession numberBC008979
Gene Symbol FANCA
Gene ID (NCBI) 2175
RRIDAB_2101786
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15360
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FANCA, also named as FAA, FACA and FANCH, is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

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