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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM96B Polyclonal antibody (20108-1-AP)

    WB analysis using 20108-1-AP (same clone as 20108-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FAM96B Polyclonal antibody (20108-1-AP)

    WB analysis of HEK-293 using 20108-1-AP (same clone as 20108-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using FAM96B Polyclonal antibody (20108-1-AP)

    WB analysis of Ramos using 20108-1-AP (same clone as 20108-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using FAM96B Polyclonal antibody (20108-1-AP)

    IP experiment of K-562 using 20108-1-AP (same clone as 20108-1-PBS)

    IP result of anti-FAM96B (IP:20108-1-AP, 4ug; Detection:20108-1-AP 1:300) with K-562 cells lysate 2400 ug. This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FAM96B Polyclonal antibody (20108-1-AP)

    IHC staining of mouse kidney using 20108-1-AP (same clone as 20108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FAM96B Polyclonal antibody (20108-1-AP)

    IHC staining of mouse kidney using 20108-1-AP (same clone as 20108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM96B Polyclonal antibody (20108-1-AP)

    IHC staining of mouse testis using 20108-1-AP (same clone as 20108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM96B Polyclonal antibody (20108-1-AP)

    IHC staining of mouse testis using 20108-1-AP (same clone as 20108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FAM96B Polyclonal antibody, PBS Only

FAM96B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 20108-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20108-1-PBS targets FAM96B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13827

Product name: Recombinant human FAM96B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-163 aa of BC001733

Sequence: MVGGGGVGGGLLENANPLIYQRSGERPVTAGEEDEQVPDSIDAREIFDLIRSINDPEHPLTLEELNVVEQVRVQVSDPESTVAVAFTPTIPHCSMATLIGLSIKVKLLRSLPQRFKMDVHITPGTHASEHAVNKQLADKERVAAALENTHLLEVVNQCLSARS

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 96, member B
Calculated molecular weight 163 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC001733
Gene Symbol FAM96B
Gene ID (NCBI) 51647
RRIDAB_10666848
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3D0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FAM96B, also known as MIP18 or CIAO2B (Cytosolic iron-sulfur assembly component 2B), is a component of the cytosolic iron-sulfur protein assembly (CIA) complex involved in iron homeostasis and part of the MMXD complex contributed to proper chromosome segregation (PMID: 36503010). FAM96B may act as a scaffold protein to recruit CKB and the other components of the MMXD complex to associate with the spindle and mitotic apparatus and contributes to chromosome segregation.

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