Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 5 publications below |
Product Information
24803-1-AP targets FAM92A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20738 Product name: Recombinant human FAM92A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 162-318 aa of BC014598 Sequence: ATLTARNREAKQLTQLERTRQRNPSDRHVISQAETELQRAAMDASRTSRHLEETINNFERQKMKDIKTIFSEFITIEMLFHGKALEVYTAAYQNIQNIDEDEDLEVFRNSLYAPDYSSRLDIVRANSKSPLQRSLSAKCVSGTGQEAGWAATCQTLI 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 92, member A1 |
| Calculated molecular weight | 289 aa, 33 kDa |
| Observed molecular weight | 29-33 kDa |
| GenBank accession number | BC014598 |
| Gene Symbol | FAM92A1 |
| Gene ID (NCBI) | 137392 |
| RRID | AB_2879735 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A1XBS5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM92A1 antibody 24803-1-AP | Download protocol |
| IHC protocol for FAM92A1 antibody 24803-1-AP | Download protocol |
| IP protocol for FAM92A1 antibody 24803-1-AP | Download protocol |
| WB protocol for FAM92A1 antibody 24803-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Biol BAR Domain-Containing FAM92 Proteins Interact with Chibby1 to Facilitate Ciliogenesis.
| ||
Life Sci Alliance ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose tolerance | ||
J Exp Clin Cancer Res Nuclear FAM289-Galectin-1 interaction controls FAM289-mediated tumor promotion in malignant glioma. | ||
Am J Hum Genet Mutations in SCNM1 cause orofaciodigital syndrome due to minor intron splicing defects affecting primary cilia | ||
Adv Sci (Weinh) The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers | ||
Nat Commun Membrane remodeling by FAM92A1 during brain development regulates neuronal morphology, synaptic function, and cognition |
