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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using FAM8A1 Polyclonal antibody (24746-1-AP)

    WB analysis of HEK-293 using 24746-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24746-1-AP (FAM8A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using FAM8A1 Polyclonal antibody (24746-1-AP)

    WB analysis of mouse kidney using 24746-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24746-1-AP (FAM8A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM8A1 Polyclonal antibody (24746-1-AP)

    IHC staining of mouse testis using 24746-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24746-1-AP (FAM8A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM8A1 Polyclonal antibody (24746-1-AP)

    IHC staining of mouse testis using 24746-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24746-1-AP (FAM8A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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FAM8A1 Polyclonal antibody

FAM8A1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24746-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AHCP, FAM8A1, Protein FAM8A1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

24746-1-AP targets FAM8A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM8A1 fusion protein Ag20476 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 8, member A1
Calculated molecular weight 413 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC047881
Gene Symbol FAM8A1
Gene ID (NCBI) 51439
RRIDAB_2879702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM8A1 antibody 24746-1-APDownload protocol
IHC protocol for FAM8A1 antibody 24746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders

Authors - Huilun Helen Wang
View Article
humanWB

Nat Commun

SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex

Authors - Liangguang Leo Lin
View Article