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Tested Applications
| Positive WB detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32472-1-AP targets FAM86A in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FAM86A fusion protein Ag38481 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 86, member A |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC010084 |
| Gene Symbol | FAM86A |
| Gene ID (NCBI) | 196483 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96G04 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM86A is a class I lysine methyltransferase (KMT), belonging to the family of protein methyltransferases (KMTs), which produces trimethylation (K525me3) at the Lys525 site of eukaryotic translation elongation factor 2 (EEF2) and promotes the binding of eEF2 to ribosomes, thereby regulating the protein synthesis. (PMID: 38508183) FAM86A is expressed in a highly dependent manner in a variety of human cancer cell lines, suggesting that FAM86A may be a potential target for future anticancer therapy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM86A antibody 32472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |