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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using FAM84B Recombinant monoclonal antibody (87176-3-RR)

    WB analysis of MCF-7 using 87176-3-RR

    WB result of FAM84B antibody (87176-3-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM84B transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using FAM84B Recombinant monoclonal antibody (87176-3-RR)

    WB analysis using 87176-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87176-3-RR (FAM84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FAM84B Recombinant monoclonal antibody (87176-3-RR)

    WB analysis using 87176-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87176-3-RR (FAM84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FAM84B Recombinant monoclonal antibody (87176-3-RR)

    IF Staining of MCF-7 using 87176-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FAM84B antibody (87176-3-RR, Clone: 252473G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87176-3-RR

    Affinity of 87176-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87176-3-RR against Human FAM84B were performed. The affinity constant is 4.50 nM.

  • KD/KO Validated

FAM84B Recombinant monoclonal antibody

FAM84B Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87176-3-RR
Clone No.252473G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

LRAT domain-containing 2, LRATD2, NSE2, Protein FAM84B, Protein LRATD2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, mouse stomach tissue, MDA-MB-453 cells, rat stomach tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87176-3-RR targets FAM84B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag13243

Product name: Recombinant human FAM84B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-310 aa of BC052957

Sequence: MGNQVEKLTHLSYKEVPTADPTGVDRDDGPRIGVSYIFSNDDEDVEPQPPPQGPDGGGLPDGGDGPPPPQPQPYDPRLHEVECSVFYRDECIYQKSFAPGSAALSTYTPENLLNKCKPGDLVEFVSQAQYPHWAVYVGNFQVVHLHRLEVINSFLTDASQGRRGRVVNDLYRYKPLSSSAVVRNALAHVGAKERELSWRNSESFAAWCRYGKREFKIGGELRIGKQPYRLQIQLSAQRSHTLEFQSLEDLIMEKRRNDQIGRAAVLQELATHLHPAEPEEGDSNVARTTPPPGRPPAPSSEEEDGEAVAH

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 84, member B
Calculated molecular weight 34 kDa
Observed molecular weight40 kDa
GenBank accession numberBC052957
Gene Symbol FAM84B
Gene ID (NCBI) 157638
RRIDAB_3745391
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96KN1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM84B (Family with sequence similarity 84 member B) is a protein-coding gene located on human chromosome 8. Although its full physiological function remains under investigation, accumulating evidence links FAM84B to cell proliferation, cytoskeletal organization and cancer progression. In several tumor types-most notably prostate, esophageal squamous-cell, breast and glioma-FAM84B is frequently amplified or over-expressed, and high expression often correlates with advanced stage, higher Gleason score and poorer patient survival.

Protocols

Product Specific Protocols
IF protocol for FAM84B antibody 87176-3-RRDownload protocol
WB protocol for FAM84B antibody 87176-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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