Validation Data Gallery
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Tested Applications
| Positive WB detected in | BxPC-3 cells, DU 145 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 8 publications below |
| IHC | See 7 publications below |
| IF | See 1 publications below |
Product Information
20618-1-AP targets FAM83A in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14680 Product name: Recombinant human FAM83A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-294 aa of BC007828 Sequence: MSRSRHLGKIRKRLEDVKSQWVRPARADFSDNESARLATDALLDGGSEAYWRVLSQEGEVDFLSSVEAQYIQAQAREPPCPPDTLGGAEAGPKGLDSSSLQSGTYFPVASEGSEPALLHSWASAEKPYLKEKSSATVYFQTVKHNNIRDLVRRCITRTSQNISIRSVEGEIYCAKSGRKFTGQIREKFIISDWRFVLSGSYSFTWLCGHVHRNILSKFTGQAVELFDEEFRHLYASSKPVMGLKSPRLVAPVPPGAAPANGRLSSSSGSASDRTSSNPFSGRSAGSHPGTRTDG 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 83, member A |
| Calculated molecular weight | 434 aa, 47 kDa |
| Observed molecular weight | 47-58 kDa |
| GenBank accession number | BC007828 |
| Gene Symbol | FAM83A |
| Gene ID (NCBI) | 84985 |
| RRID | AB_2878708 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UY5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM83A antibody 20618-1-AP | Download protocol |
| WB protocol for FAM83A antibody 20618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Oncol (Dordr) Genomic analysis and filtration of novel prognostic biomarkers based on metabolic and immune subtypes in pancreatic cancer | ||
Int J Mol Sci Involvement of MicroRNA-1-FAM83A Axis Dysfunction in the Growth and Motility of Lung Cancer Cells.
| ||
Clin Epigenetics Aberrant epigenetic and transcriptional events associated with breast cancer risk. | ||
J Transl Med FAM83A promotes proliferation and metastasis via Wnt/β-catenin signaling in head neck squamous cell carcinoma.
| ||
BMC Cancer Lovastatin inhibits erythroleukemia progression through KLF2-mediated suppression of MAPK/ERK signaling |
