Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, U2OS cells, human testis tissue, rat liver |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33752-1-AP targets FAM82A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40522 Product name: Recombinant human FAM82A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 29-125 aa of BC024243 Sequence: KVRKPGIAMKLPEFLSLGNTFNSITLQDEIHDDQGTTVIFQERQLQILEKLNELLTNMEELKEEIRFLKEAIPKLEEYIQDELGGKITVHKISPQHR 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 82, member A1 |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC024243 |
| Gene Symbol | FAM82A1 |
| Gene ID (NCBI) | 151393 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96LZ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM82A1 is a crucial cellular signaling regulatory protein that has recently gained attention for its role in tumorigenesis. Research indicates that it serves as an important regulatory subunit of protein phosphatase 2A (PP2A), participating in the precise regulation of cell cycle progression and apoptosis by influencing key signaling pathways such as JNK/c-Jun. In various cancers (e.g., glioblastoma, lung cancer), FAM82A1 often exhibits abnormally high expression, which is closely associated with accelerated cancer cell proliferation, inhibited apoptosis, and poor patient prognosis. Its cancer-promoting function is primarily achieved by sustaining cancer cell survival, making FAM82A1 not only a potential tumor biomarker but also a promising new target for anti-cancer therapy worthy of further exploration.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM82A1 antibody 33752-1-AP | Download protocol |
| IHC protocol for FAM82A1 antibody 33752-1-AP | Download protocol |
| IP protocol for FAM82A1 antibody 33752-1-AP | Download protocol |
| WB protocol for FAM82A1 antibody 33752-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |