Validation Data Gallery
Tested Applications
| Positive WB detected in | J774A.1 cells, SH-SY5Y cells, SK-N-SH cells, U-937 cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32882-1-AP targets FAM76B in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36658 Product name: Recombinant human FAM76B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 179-277 aa of NM_144664.4 Sequence: HHRHSSSHHKISNLSPEEEQGLWKQSHKSSATIQNETPKKKPKLESKPSNGDSSSINQSADSGGTDNFVLISQLKEEVMSLKRLLQQRDQTILEKDKKL 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 76, member B |
| Calculated molecular weight | 39kDa，339aa |
| Observed molecular weight | 36-39 kDa |
| GenBank accession number | NM_144664.4 |
| Gene Symbol | FAM76B |
| Gene ID (NCBI) | 143684 |
| RRID | AB_3742775 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q5HYJ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM76B is a nuclear protein with a molecular weight of about 39 kDa. Its amino acid sequence is highly conserved, and it is highly similar to FAM76B sequence of rats, mice and zebrafish. The protein contains histidine repeat sequence (poly-His domain) and ubiquitination site of Lys-225. FAM76B inhibits the inflammatory reaction mediated by NF-κB by affecting the cytoplasmic translocation of hnRNPA2B1. The expression of inflammatory factors (such as IL-6) increased significantly in FAM76B gene knockout mice, indicating that FAM76B has anti-inflammatory effect.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM76B antibody 32882-1-AP | Download protocol |
| WB protocol for FAM76B antibody 32882-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |