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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using FAM71F2 Polyclonal antibody (24286-1-AP)

    WB analysis of PC-3 using 24286-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24286-1-AP (FAM71F2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using FAM71F2 Polyclonal antibody (24286-1-AP)

    WB analysis of mouse liver using 24286-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24286-1-AP (FAM71F2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using FAM71F2 Polyclonal antibody (24286-1-AP)

    IF Staining of PC-3 using 24286-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 24286-1-AP (FAM71F2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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FAM71F2 Polyclonal antibody

FAM71F2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24286-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Protein FAM71F2, Protein FAM137B, Golgi-associated RAB2 interactor protein 1A, GARIN1A, FAM137B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, mouse liver tissue
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24286-1-AP targets FAM71F2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM71F2 fusion protein Ag16874 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 71, member F2
Calculated molecular weight 309 aa, 35 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC105730
Gene Symbol FAM71F2
Gene ID (NCBI) 346653
RRIDAB_2879473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NXP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM71F2 antibody 24286-1-APDownload protocol
IF protocol for FAM71F2 antibody 24286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols