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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM71E2 Polyclonal antibody

FAM71E2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20904-1-AP

Synonyms

C19orf16, FAM71E2, Protein FAM71E2

Validation Data Gallery

Applications:
  • WB analysis of human brain using 20904-1-AP

    WB analysis of human brain using 20904-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20904-1-AP (FAM71E2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 20904-1-AP

    WB analysis of HepG2 using 20904-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20904-1-AP (FAM71E2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20904-1-AP

    IHC staining of human brain using 20904-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20904-1-AP (FAM71E2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 20904-1-AP

    IHC staining of human brain using 20904-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20904-1-AP (FAM71E2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 20904-1-AP

    IHC staining of human testis using 20904-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20904-1-AP (FAM71E2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 20904-1-AP

    IHC staining of human testis using 20904-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20904-1-AP (FAM71E2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman brain tissue, HepG2 cells
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20904-1-AP is FAM71E2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM71E2 fusion protein Ag15027 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 71, member E2
Calculated molecular weight 302 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC031875
Gene symbol FAM71E2
Gene ID (NCBI) 284418
RRIDAB_11183765
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM71E2 antibody 20904-1-APDownload protocol
IHC protocol for FAM71E2 antibody 20904-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols