Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cells, U-87 MG cells, SK-BR-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32562-1-AP targets FAM50B in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38226 Product name: Recombinant human FAM50B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 73-141 aa of NM_012135.2 Sequence: MKARQEALVRERERQLAKRQHLEEQRLQQERQREQEQRRERKRKISCLSFALDDLDDQADAAEARRAGN 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 50, member B |
| Calculated molecular weight | 39kDa，325aa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | NM_012135.2 |
| Gene Symbol | FAM50B |
| Gene ID (NCBI) | 26240 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y247 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM50B, also known as family with sequence similarity 50, member B, is a protein-coding gene located on chromosome 6. It is widely expressed in various tissues, with particularly high abundance in the testis and both adult and fetal brain. The exact function of FAM50B is not yet fully understood and is still under investigation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM50B antibody 32562-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |