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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM48A Polyclonal antibody

FAM48A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24419-1-AP

Synonyms

C13, C13orf19, FAM48A, FP757, p38 interacting protein, P38IP, Protein FAM48A

Validation Data Gallery

Applications:
  • WB analysis of PC-3 using 24419-1-AP

    WB analysis of PC-3 using 24419-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 24419-1-AP

    WB analysis of PC-3 using 24419-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 24419-1-AP

    WB analysis of Jurkat using 24419-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24419-1-AP

    IHC staining of human testis using 24419-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24419-1-AP (FAM48A Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with SUPT20H/FAM48A antibody included
  • IHC staining of human testis using 24419-1-AP

    IHC staining of human testis using 24419-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24419-1-AP (FAM48A Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SUPT20H/FAM48A antibody included

Tested Applications

Positive WB detected inPC-3 cells, Jurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SUPT20H/FAM48A Ready-To-Use IHC Kit. SUPT20H/FAM48A primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24419-1-AP is FAM48A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM48A fusion protein Ag19875 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 48, member A
Calculated molecular weight 779 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC030686
Gene symbol FAM48A
Gene ID (NCBI) 55578
RRIDAB_2879537
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM48A also named as C13orf19 or SUPT20H is a 779 amino acid protein, which belongs to the SPT20 family. FAM48A is required for MAP kinase p38 activation during gastrulation and for starvation-induced ATG9A trafficking during autophagy. FAM48A localizes in the nucleus and is highly expressed in testis, moderately in brain and pituitary gland.

Protocols

Product Specific Protocols
WB protocol for FAM48A antibody 24419-1-APDownload protocol
IHC protocol for FAM48A antibody 24419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols