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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using FAM29A Polyclonal antibody (16933-1-AP)

    WB analysis of Jurkat using 16933-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16933-1-AP (FAM29A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using FAM29A Polyclonal antibody (16933-1-AP)

    WB analysis of MCF-7 using 16933-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16933-1-AP (FAM29A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FAM29A Polyclonal antibody (16933-1-AP)

    WB analysis of K-562 using 16933-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16933-1-AP (FAM29A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FAM29A Polyclonal antibody (16933-1-AP)

    IF Staining of HeLa using 16933-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FAM29A antibody (16933-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FAM29A Polyclonal antibody (16933-1-AP)

    IF Staining of HeLa using 16933-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM29A antibody (16933-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FAM29A Polyclonal antibody

FAM29A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16933-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA1574, HAUS6, HAUS augmin-like complex subunit 6, DGT6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16933-1-AP targets FAM29A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10421

Product name: Recombinant human FAM29A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-354 aa of BC010632

Sequence: MEENRTKEPIQMDVEHREVLPESLPVLHNQREFSMADFLLETTVSDFGQSHLTEEKVISDCECVPQKHVLTSHIDEPPTQNQSDLLNKKVICKQDLECLAFTKLSETSRMETFSPAVGNRIDVMGGSEEEFMKILDHLEVSCNKPSTNKTMLWNSFQISIGISSKSFKDNDFGILHETLPEEVGHLSFNSSSSSEANFKLEPNSPMHGGTLLEDVVGGRQTTPESDFNLQALRSRYEALKKSLSKKREESYLSNSQTPERHKPELSPTPQNVQTDDTLNFLDTCDLHTEHIKPSLRTSIGERKRSLSPLIKFSPVEQRLRTTIACSLGELPNLKEEDILNKSLDAKEPPSDLTR

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 29, member A
Calculated molecular weight 955 aa, 109 kDa
Observed molecular weight 109 kDa
GenBank accession numberBC010632
Gene Symbol FAM29A
Gene ID (NCBI) 54801
RRIDAB_1939544
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4H7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for FAM29A antibody 16933-1-APDownload protocol
WB protocol for FAM29A antibody 16933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article
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