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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM21C Polyclonal antibody (33047-1-AP)

    WB analysis using 33047-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33047-1-AP (FAM21C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FAM21C Polyclonal antibody (33047-1-AP)

    IF Staining of A431 using 33047-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FAM21C antibody (33047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FAM21C Polyclonal antibody

FAM21C Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 33047-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0592, Vaccinia virus penetration factor, VPEF, WASH complex subunit 2C, WASHC2C

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HuH-7 cells, L02 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33047-1-AP targets FAM21C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15066

Product name: Recombinant human FAM21C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1036-1318 aa of BC006456

Sequence: MSVPRGPIAQWADGAISPNGHRPQLRAASGEDSTEEALAAAAAPWEGGPVPGVDTSPFAKSLGHSRGEADLFDSGDIFSTGTGSQSVERTKPKAKIAENPANPPVGGKAKSPMFPALGEASSDDDLFQSAKPKPAKKTNPFPLLEDEDDLFTDQKVKKNETKSSSQQDVILTTQDIFEDDIFATEAIKPSQKTREKEKTLESNLFDDNIDIFADLTVKPKEKSKKKVEAKSIFDDDMDDIFSTGIQAKTTKPKSRSAQAAPEPRFEHKVSNIFDDPLNAFGGQ

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 21, member C
Calculated molecular weight 1320 aa, 145 kDa
Observed molecular weight200 kDa
GenBank accession numberBC006456
Gene Symbol FAM21C
Gene ID (NCBI) 253725
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9Y4E1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for FAM21C antibody 33047-1-APDownload protocol
WB protocol for FAM21C antibody 33047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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