Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 2 publications below |
Product Information
25395-1-AP targets FAM20C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FAM20C fusion protein Ag22061 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 20, member C |
| Calculated molecular weight | 584 aa, 66 kDa |
| GenBank accession number | BC040074 |
| Gene Symbol | FAM20C |
| Gene ID (NCBI) | 56975 |
| RRID | AB_2880058 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IXL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM20C, also named as Golgi-enriched fraction casein kinase or Dentin matrix protein 4, is a 584 amino acid protein, which belongs to the FAM20 family. FAM20C is widely expressed. FAM20C as a Calcium-binding kinase that phosphorylates the caseins and several secreted proteins implicated in biomineralization, including the secretory calcium binding phosphoproteins (SCPP).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM20C antibody 25395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Altered sulfation status of FAM20C-dependent chondroitin sulfate is associated with osteosclerotic bone dysplasia | ||
FASEB J Fam20C-mediated phosphorylation of osteopontin is critical for its secretion but dispensable for its action as a cytokine in the activation of hepatic stellate cells in liver fibrogenesis. | ||
Gene Knocking out FAM20C in pre-osteoblasts leads to up-regulation of osteoclast differentiation to affect long bone development | ||
Int J Biol Macromol Discovery of a novel Fam20C inhibitor for treatment of triple-negative breast cancer |