• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM20A Polyclonal antibody

FAM20A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25258-1-AP

Synonyms

FAM20A, FP2747, Protein FAM20A

Validation Data Gallery

Applications:
  • WB analysis of mouse lung using 25258-1-AP

    WB analysis of mouse lung using 25258-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25258-1-AP (FAM20A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 25258-1-AP

    IHC staining of human colon cancer using 25258-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25258-1-AP (FAM20A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary using 25258-1-AP

    IHC staining of human ovary using 25258-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25258-1-AP (FAM20A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 25258-1-AP

    IHC staining of human ovary using 25258-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25258-1-AP (FAM20A Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon cancer using 25258-1-AP

    IHC staining of human colon cancer using 25258-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25258-1-AP (FAM20A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman colon cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25258-1-AP is FAM20A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM20A fusion protein Ag19494 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 20, member A
Calculated molecular weight 541 aa, 61 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC136686
Gene symbol FAM20A
Gene ID (NCBI) 54757
RRIDAB_2879991
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM20A antibody 25258-1-APDownload protocol
IHC protocol for FAM20A antibody 25258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols