Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, U2OS cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21078-1-AP targets FAM190B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15281 Product name: Recombinant human KIAA1128 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-354 aa of BC030528 Sequence: MPNGTPVNLLGTSKNSNVKSYIKNNGSDCPSSHSFNWRKANKYQLCAQGVEEPNNTQNSHDKIIDPEKRVPTQGMFDKNGIKGGLKSVSLFTSKLAKPSTMFVSSTEELNQKSFSGPSNLGKFTKGTLLGRTSYSSINTPKSQLNGFYGNRSAGSMQRPRANSCATRSSSGESLAQSPDSSKSINCEKMVRSQSFSHSIQNSFLPPSSITRSHSFNRAVDLTKPYQNQQLSIRVPLRSSMLTRNSRQPEVLNGNEHLGYGFNRPYAAGGKKLALPNGPGVTSTLGYRMVHPSLLKSSRSPFSGTMTVDGNKNSPADTCVEEDATVLAKDRAANKDQELIENESYRTKNNQTMKH 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA1128 |
| Calculated molecular weight | 834 aa, 94 kDa |
| Observed molecular weight | 63 kDa |
| GenBank accession number | BC030528 |
| Gene Symbol | KIAA1128 |
| Gene ID (NCBI) | 54462 |
| RRID | AB_3742014 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H7U1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM190B (Family with sequence similarity 190, member B), also known as Gcap14, CCSER2, KIAA1128, NPD012, is a CC domain-containing protein with multiple isoforms generated by alternative splicing that functions as an MAP. FAM190B is a microtubule plus-end-tracking protein and as a regulator of microtubule dynamics during neurodevelopment (PMID: 36795749).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM190B antibody 21078-1-AP | Download protocol |
| WB protocol for FAM190B antibody 21078-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |