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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using FAM185A Recombinant antibody (83921-3-RR)

    WB analysis of HT-29 using 83921-3-RR (same clone as 83921-3-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 83921-3-RR (FAM185A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83921-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using FAM185A Recombinant antibody (83921-3-RR)

    IHC staining of human ovarian cancer using 83921-3-RR (same clone as 83921-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83921-3-RR (FAM185A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83921-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83921-3-RR

    Affinity and Kinetic Characterization of 83921-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83921-3-RR against Human FAM185A were performed. The affinity constant is below 1 pM.

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FAM185A Recombinant antibody, PBS Only

FAM185A Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83921-3-PBS
Clone No.240804F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

240804F7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83921-3-PBS targets FAM185A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FAM185A fusion protein Ag15041 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 185, member A
Calculated molecular weight 393 aa, 42 kDa
Observed molecular weight45 kDa
GenBank accession numberBC029175
Gene Symbol FAM185A
Gene ID (NCBI) 222234
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8N0U4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.